This article details a method for collecting peripheral blood samples from mice through a tail-snipping procedure. The process ensures minimal discomfort to the animal while allowing for the collection of blood for further analysis.
Position a mouse within a disinfected chamber to ensure access to its tail.
Secure the chamber to minimize mouse movement.
Sterilize the tail and anesthetize it.
Using sharp surgical scissors, snip off a small portion from the tip of the mouse's tail, which contains a network of blood vessels.
This tail-snipping procedure enables the collection of a small volume of peripheral blood.
Collect the blood into a heparinized capillary tube using the tube's capillary action. The heparinized tube helps prevent blood coagulation.
Using a bulb syringe, transfer the blood into an EDTA-coated tube.
Invert the tube for proper mixing of the blood with EDTA, an anticoagulant. EDTA binds calcium ions within the blood, preventing coagulation.
Centrifuge the tube to separate the plasma from the blood cells.
Collect and transfer the plasma-containing top layer to a fresh tube.
Store the tube at lower temperatures to maintain the integrity of the plasma components.
To collect a peripheral blood sample, first, gently position the mouse in a disinfected small animal restrainer, and secure the tube to minimize the animal's movements.
Next, scrub the tail with fresh isopropyl alcohol, followed by the application of topical anesthetic cream.
Using very sharp surgical scissors, snip about 0.5 to 1 millimeter from the tip of the tail, and collect approximately 80 microliters of peripheral blood into heparinized capillary tubes.
Use a bulb syringe to transfer the blood samples into labeled potassium-EDTA microtainer tube, and invert the tube 20 times to prevent coagulation.
After centrifuging blood collection tubes per manufacturer's instructions, carefully collect the plasma layer and store the aliquots at minus 20 degrees Celsius, and aliquot the plasma for minus 20 degrees Celsius storage.
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