This study outlines a method for recording electroretinogram (ERG) and visual evoked potential (VEP) signals in rats. The procedure involves surgically implanting electrodes and ensuring controlled conditions for accurate measurements.
Begin with a rat with surgically implanted electrodes in its eye and brain.
The electroretinogram, or ERG electrode, measures the electrical activity of the retina, while the visual evoked potential, or VEP electrodes, record brain signals.
These electrodes are connected to a transmitter implanted in the rat's abdomen.
Dark-adapt the animal before the recordings.
Perform the procedure under dim red light.
Apply anesthetic and dilating drops to the cornea.
Guide the animal into a restrainer to minimize head movement.
Position the animal in front of the Ganzfeld bowl, which ensures uniform exposure of the retina under controlled illumination.
Align the animal's eyes with the opening of the bowl.
Pass a magnet over the implanted transmitter to turn it on. Confirm its activation on the receiver.
Record ERG and VEP signals under different illumination conditions.
For recording dark-adapted signals, animals are dark-adapted for 12 hours, and all procedures are performed in a dark room with the aid of dim red light. For illustration purposes, recordings will be conducted under normal room lighting conditions here.
Before recording, apply topical anesthesia and dilating drops to the cornea of the rat. Next, guide the conscious animal into a custom-made clear restrainer. Place the rat in front of the Ganzfeld bowl with its eyes aligned with the opening of the bowl.
Turn on the indwelling transmitter by passing a magnet within 5 centimeters of it. Verify that the transmitter is on by checking the LED status light on the receiver base. Then, collect signals over a range of luminous energies.
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