This study investigates the effects of a chemokine antagonist on glucose metabolism in mice. By using a micro-osmotic pump to deliver the drug directly into the brain, researchers can assess the impact on insulin signaling and glucose tolerance.
Begin with a mouse implanted with a micro-osmotic pump, which continuously delivers a drug directly into the brain over a set period.
This drug is an antagonist of a chemokine that binds to its receptor, blocking hypothalamic insulin signaling and impairing glucose metabolism.
Fast the mouse for the desired time interval.
Pick up the mouse and stroke the tail to ensure sufficient blood flow.
Cut off a small portion of the tail to obtain a drop of blood.
Place the blood drop onto the glucose chip inserted into a glucometer and record the glucose level.
Then, feed the mouse a glucose solution orally.
Measure the glucose levels at desired time intervals.
Persistently high glucose levels show impaired glucose metabolism by the drug.
Before starting the glucose tolerance test, prepare the glucose solution by dissolving 3.75 grams glucose and 15 milliliters distilled water. Set up a time table with proper intervals between each blood examination to record the readings during the experiment. Next, weigh each mouse after fasting to calculate the appropriate amount of glucose for the injection. Then at the workbench, prepare timer, glucose chip, glucometer, insulin syringe, and razor blades.
To measure the blood glucose level, first insert a new glucose chip into the glucometer and press the Start button to set the 0. Then, pick up the mouse at the back of the neck and stroke its tail to ensure sufficient blood flow. Use a new razor blade to cut off a small piece of the tail, and then squeeze out a drop of blood into the glucose chip. Using intragastric gavage technique, feed the mouse glucose and immediately start the timer. Measure the glucose at different time points.
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