This study investigates the effects of prolonged high-volume noise exposure on auditory perception in mice. It highlights the mechanisms of noise-induced hearing loss through the damage of hair cells in the cochlea.
Start with a cushioned rat trap cage divided into four compartments, each housing a mouse.
Place this cage inside a soundproof box.
Position the speaker in front of the cage and a microphone above it to monitor the sound level.
Close the soundproof box to isolate the mice from external noise.
Expose the mice to high-volume noise for an extended period over several days.
As sound waves enter the ear, they travel to the cochlea, where hair-like projections in the hair cells detect vibrations caused by the sound wave.
This excites the hair cells and transmits the signal to connected auditory nerve fibers, generating signals that travel to the brain for sound perception.
However, prolonged exposure to high-volume noise damages the hair-like projections, reducing their ability to detect sound waves.
This decreases the signal transmission to auditory nerves and the brain, diminishing sound perception and resulting in noise-induced hearing loss.
Place four male C57 black 6J mice into the cage, one for each quarter for noise exposure. Randomly assign the mice to each quarter during the noise exposure. Place a microphone on the top of the cage to monitor the noise level during the noise exposure.
Place the cage in front of the speaker inside a soundproof box, ensuring the speaker is positioned 8.5 centimeters away from the cage. Expose the mice to the noise at frequencies of 1 kilohertz and 6 kilohertz continuously for six hours per day, for five consecutive days.
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