This study investigates the effects of low-intensity infrared laser light on the blood-brain barrier (BBB) in a mouse model of glioblastoma. The laser induces oxidative stress that disrupts tight junctions, enhancing BBB permeability and facilitating drug delivery to the tumor.
Begin with a mouse model of glioblastoma, an invasive brain tumor.
At the tumor periphery, the blood-brain barrier (BBB) remains intact, with endothelial cells forming tight junctions that regulate molecular entry.
The mouse is fitted with a head-mounted optical fiber to deliver laser light.
Administer a low-intensity infrared laser, which penetrates the skin and skull to reach the brain.
The laser excites molecular oxygen in brain cells, generating reactive oxygen species (ROS).
The resulting oxidative stress disrupts tight junctions and increases BBB permeability.
Fluid leakage through the BBB enhances the flow of interstitial fluid (ISF), which surrounds brain cells and blood vessels.
Intravenously inject a suspension of liposomes, which are nano-sized vesicles designed for drug delivery.
The liposomes cross the disrupted BBB, and the enhanced ISF flow facilitates their distribution and accumulation within the tumor.
Over time, the expression of tight junction proteins restores BBB integrity and prevents further leakage.
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