This study investigates the effects of various treatments on neuroblastoma and microglia cells using a transwell system. It explores the neuroprotective potential of photobiomodulation therapy through different laser wavelengths.
Divide the transwell system into five groups that undergo various treatments.
The first group serves as the control and consists of a co-culture of neuroblastoma and microglia cells.
In the second group, treat the co-culturer with fibrillar β-amyloid (fAβ) alone. fAβ induces damage in neuroblastoma cells.
In the third group, treat neuroblastoma cells with an 808-nanometer laser, then co-culture them with untreated microglia.
The laser reduces oxidative stress, improving neuronal survival.
In the fourth group, treat microglia with a 1,064-nanometer laser, then co-culture them with untreated neuroblastoma cells.
Activated microglia enhance fAβ clearance and release cytokines, protecting neuroblastoma cells.
In the fifth group, expose microglia to a 1,064 nanometer laser and neuroblastoma cells to an 808 nanometer laser, then co-culture them.
The combined effects of dual-wavelength exposure lead to greater neuronal survival compared to single-wavelength treatments, demonstrating the neuroprotective potential of synergistic photobiomodulation therapy.
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