This study investigates the use of a degradable nanostructured porous silicon film for the sustained release of nerve growth factor (NGF) to promote the survival and neurite growth of PC12 cells. The method involves loading NGF into the silicon film and observing its effects on cell behavior over time.
Begin with rat pheochromocytoma 12 or PC12 cells in basic growth media.
Take a collagen-coated well containing differentiation media and place the cells in it.
Incubate to facilitate cell adhesion.
Take a degradable nanostructured porous silicon or PSi film.
The pores of the film are loaded with nerve growth factor or NGF, which is adsorbed through electrostatic interactions between the negatively charged NGF and the positively charged PSi surface.
Place the NGF-loaded PSi film inside the well and incubate.
Change the media at regular intervals.
The NGF within the PSi film is released into the media by diffusion and when the silicon scaffold degrades.
This delivery system enables a sustained release of NGF at a controlled rate over an extended period.
NGFs interact with the PC12 cell, enabling its survival and facilitating neurite growth and branching.
Seed one times 10 to the fourth cells per centimeter squared working area on a colligen type one coated plates in the presence of differentiation medium. Place the plates in the incubator.
After 24 hours at 37 degrees Celsius add fresh murine beta NGF or NGF loaded porous silicon carrier to each plate and return the plates to the incubator.
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