This study investigates the role of endophytic bacteria in promoting tomato plant growth under salt stress conditions. By comparing inoculated and control seedlings, the research highlights the beneficial effects of these bacteria on plant health.
Endophytic bacteria colonize plant tissues and promote plant growth.
To study their impact on tomato plant growth under salt stress, start with sterile seedlings showing developing leaves and roots.
Immerse the roots of the experimental seedling in a suspension of endophytic bacteria; leave the control uninoculated.
Transfer both groups into sterilized soil to prevent external microbial interference and incubate in a growth chamber.
From the soil, the bacteria enter through transient root openings, colonize the internal tissues, and spread to other plant parts. Reapply the bacterial suspension to reinforce colonization.
Apply a sodium chloride solution on alternate days to induce progressive salt stress in both groups.
Salt stress triggers the production of reactive oxygen species (ROS), which damage plant cells.
Endophytes mitigate stress by producing ROS-scavenging enzymes and supplying phytohormones and micronutrients for plant growth.
Post-incubation, the experimental seedling maintains growth, while the control exhibits arrested development.
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