Fluorescence molecular tomography (FMT) is an advanced imaging technique used to visualize the distribution of fluorescent proteins in tumors, particularly glioblastoma. This method involves the use of near-infrared light to penetrate brain tissue, allowing for the detection and imaging of cancerous cells.
Fluorescence molecular tomography or FMT is an imaging technique that helps determine the distribution of fluorescent proteins present in tumors. To perform FMT, begin by taking an anesthetized mouse. This mouse model is engineered to express near-infrared fluorescent proteins in the glioblastoma cells - the cancerous cells of the brain.
Now, place the mouse in the prone position in the center of an imaging cassette. Fasten the cassette lid and insert it into the docking station of an FMT imager. Once inside, identify an appropriate tumor area containing labeled glioblastoma cells. The near-infrared light penetrates deeply through the brain tissue reaching the target cells.
These light waves scan the entire tumor area at multiple points. Consequently, the fluorescent proteins within the glioblastoma cells produce fluorescent emission signals. These signals are then detected by the transillumination system from above, which generates high-quality 2D images from various locations within the selected tumor area.
Finally, use the software algorithms to combine these images and reconstruct a 3D-tomographic model that indicates tumor localization in the brain. After scanning, remove the mouse from the imaging cassette and allow it to recover.
To image the injected cells, place the anesthetized recipient animal in the imaging cassette of a fluorescence molecular tomography imager - head adapter first in the prone position, with the head in the center of the cassette. With the cassette closed, tighten the adjustment knobs to 17 millimeters.
When the animal is secure, insert the cassette into the internal docking station and open the imager and analyzer software. In the "Experimental tab" window, select the appropriate "Database" and "Study group" and open the "Scan tab" window. Click "Select subject" to select the subject to image and select the laser channel in the "Laser channel" panel.
Click "Capture" to acquire an image, and click and drag the scan field in the captured image to identify the source locations. Check the "Add to reconstruction queue" option and click "Scan" in the "Scan tab" window. When the scanning is complete, remove the imaging cassette from the docking station and return the animal to its cage, with monitoring, until full recovery.
To analyze the images in the imager and analyzer software, open the "Analysis tab" window and click the "+" sign button in the "Dataset selection" panel to load the dataset and subject for analysis. Then, use the ellipsoid icon to select the region of interest and right-click the "Threshold" column to adjust the threshold to 0 in the "Statistic data" panel.
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