Cardiac Stress Test Induced by Dobutamine and Monitored by Cardiac Catheterization in Mice

Overview

This article describes a protocol for performing a cardiac stress test induced by dobutamine in normal mice, monitored by cardiac catheterization. It also demonstrates the application of this method to reveal subclinical cardiac disease in obese mice on a high-fat diet.

Key Study Components

Area of Science

• Cardiology
• Mouse Models
• Cardiac Catheterization

Background

• Cardiac stress tests are essential for assessing heart function.
• Dobutamine is commonly used to induce stress in cardiac studies.
• High-fat diets can lead to obesity and related cardiac issues.
• Monitoring techniques like cardiac catheterization provide detailed insights into cardiovascular health.

Purpose of Study

• To establish a reliable protocol for cardiac stress testing in mice.
• To investigate the effects of obesity on cardiac function.
• To identify subclinical cardiac diseases in high-fat diet-induced obese mice.

Methods Used

• Dobutamine-induced cardiac stress testing.
• Cardiac catheterization for monitoring arterial pressure.
• Use of anesthetized mice placed on a thermally regulated plate.
• Oxygen supply and body temperature monitoring during the procedure.

Main Results

• The protocol effectively induced cardiac stress in normal mice.
• Subclinical cardiac disease was identified in obese mice.
• Cardiac catheterization provided accurate measurements of arterial pressure.
• The method is reproducible and can be applied in various studies.

Conclusions

• The dobutamine-induced cardiac stress test is a valuable tool for cardiac research.
• This protocol can help in understanding obesity-related cardiac conditions.
• Future studies can utilize this method to explore further cardiovascular implications.

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