Murine Spinotrapezius Model to Assess the Impact of Arteriolar Ligation on Microvascular Function and Remodeling

作者： Alexander Michael Guendel*1, Kyle S. Martin*1, Joshua Cutts2, Patricia L. Foley3, Alexander M. Bailey1, Feilim Mac Gabhann4, Trevor R. Cardinal2, Shayn M. Peirce1 1Department of Biomedical Engineering,University of Virginia, 2Department of Biomedical Engineering,California Polytechnic State University, 3Office of Animal Welfare,University of Virginia, 4Department of Biomedical Engineering & Institute for Computational Medicine,Johns Hopkins University

简介：

Overview

This article presents a novel arterial ligation model in murine spinotrapezius muscle, detailing the surgical procedure and necessary instrumentation. It focuses on vascular network remodeling and functional vasodilation, utilizing intravital and confocal microscopy.

Key Study Components

Area of Science

• Neuroscience
• Vascular Biology
• Microvascular Research

Background

• Peripheral vascular disease affects a significant portion of the population.
• The model aims to study skeletal muscle ischemia through arterial ligation.
• It provides insights into structural adaptations in microvascular networks.
• The model is complementary to existing hind limb ischemia models.

Purpose of Study

• To develop a model for studying ischemic disease effects on microvascular growth.
• To visualize arterial ligation effects across the microvascular bed.
• To demonstrate functional vasodilation procedures in the spinotrapezius muscle.

Methods Used

• Arterial ligation of feeder arteries in the spinotrapezius muscle.
• Intravital microscopy for functional vasodilation assessment.
• Confocal microscopy for imaging vascular changes.
• Detailed surgical procedure with specific instrumentation.

Main Results

• Successful demonstration of the arterial ligation model.
• Visualization of microvascular adaptations post-ligation.
• Insights into functional vasodilation mechanisms.
• Establishment of a reproducible model for future research.

Conclusions

• The spinotrapezius ligation model is effective for studying ischemic conditions.
• It allows for detailed observation of vascular remodeling.
• This model can enhance understanding of vascular reactivity in ischemic diseases.

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