简介：
Overview
This article presents a novel, minimally invasive technique for creating orthotopic bladder cancer xenografts using ultrasound guidance. The method aims to reduce the morbidity associated with traditional laparotomy procedures while ensuring rapid and precise inoculation of tumor cells.
Key Study Components
Area of Science
- Oncology
- Urology
- Biomedical Imaging
Background
- Traditional methods for inoculating bladder cancer xenografts involve laparotomy, which is technically challenging and time-consuming.
- These procedures can cause significant morbidity in animal models.
- Ultrasound guidance offers a potential solution for a more efficient approach.
- Auto xenografts are critical for preclinical studies of novel bladder cancer treatments.
Purpose of Study
- To develop a rapid and precise method for creating orthotopic bladder cancer xenografts.
- To minimize invasiveness and improve the overall welfare of research animals.
- To facilitate the monitoring of tumor growth using ultrasound imaging and bioluminescence.
Methods Used
- Expansion of tumor cell lines and preparation of a cell suspension in Matrigel.
- Ultrasound visualization of the bladder and separation of its layers using saline injection.
- Injection of tumor cell suspension into the created space within the bladder.
- Monitoring tumor growth through ultrasound imaging and bioluminescence analysis.
Main Results
- The new technique was successfully performed on 50 animals with no intra- or post-interventional complications.
- Tumor growth was detected in all animals by ultrasound by day three.
- 98% of the mice exhibited continuous tumor growth during follow-up.
- Histological examination confirmed muscle invasion and some lymph node metastases.
Conclusions
- The percutaneous ultrasound-guided method is efficient and minimizes animal suffering.
- Familiarity with ultrasound imaging is essential for successful execution.
- This technique allows for ongoing monitoring of tumor dynamics and treatment responses.
What is the main advantage of the new technique?
The main advantage is that it is rapid, precise, and minimally invasive compared to traditional laparotomy methods.
How are tumor cells prepared for injection?
Tumor cells are expanded in DMEM with 10% FBS and suspended in Matrigel before injection.
What imaging technique is used to monitor tumor growth?
Ultrasound imaging and bioluminescence are used to monitor tumor growth.
How long does the procedure take once mastered?
Once mastered, the procedure can be completed in approximately three minutes.
What types of tumors were inoculated in this study?
Three different tumor cell lines were inoculated in the study.
What were the outcomes of the tumor growth monitoring?
Tumor growth was detected in all animals by day three, with 98% showing continuous growth.
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