In Vivo Optical Imaging of Brain Tumors and Arthritis Using Fluorescent SapC-DOPS Nanovesicles

作者： Zhengtao Chu1,2, Kathleen LaSance3, Victor Blanco1, Chang-Hyuk Kwon5,6, Balveen Kaur5,6, Malinda Frederick4, Sherry Thornton4, Lisa Lemen3, Xiaoyang Qi1,2 1Division of Hematology-Oncology, Department of Internal Medicine,University of Cincinnati College of Medicine, 2Division of Human Genetics,University of Cincinnati College of Medicine, 3Department of Radiology,University of Cincinnati College of Medicine, 4Division of Rheumatology, Cincinnati Children's Hospital Medical Center,University of Cincinnati College of Medicine, 5Solid Tumor Biology Program, James Comprehensive Cancer Center,The Ohio State University Medical Center, 6Department of Neurological Surgery, James Comprehensive Cancer Center,The Ohio State University Medical Center

简介：

Overview

This study presents a multi-angle rotational optical imaging (MAROI) system for in vivo quantitation of fluorescent markers in mouse models of cancer and arthritis. The MAROI technique allows for precise mapping and biological characterization of disease processes through fluorescence analysis.

Key Study Components

Area of Science

• Neuroscience
• Imaging Techniques
• Fluorescence Analysis

Background

• Fluorescent markers are crucial for imaging in biological research.
• Existing methods like 2D planar fluorescence imaging have limitations.
• In vivo imaging techniques can enhance the understanding of disease processes.
• The MAROI system aims to improve the accuracy of fluorescence imaging.

Purpose of Study

• To develop a method for optimal angle determination in fluorescence imaging.
• To assess the targeting of fluorescent markers in disease models.
• To enhance the reliability of imaging data in cancer and arthritis research.

Methods Used

• Preparation of mouse models for brain tumors and arthritis.
• Production and injection of saposin C (SapC)-DOPS nanovesicles.
• Fluorescence and X-ray imaging at various angles using the MAROI system.
• Analysis of fluorescence intensity and optimal imaging angles.

Main Results

• The MAROI method successfully identified optimal imaging angles for fluorescence analysis.
• Fluorescent signals were shown to correlate with tumor growth and disease progression.
• Small deviations from optimal angles significantly affected signal intensity.
• The technique demonstrated effective targeting of arthritic joints and tumors.

Conclusions

• The MAROI system provides a robust method for in vivo imaging in research.
• Optimal angle determination is critical for accurate fluorescence measurements.
• This technique has potential implications for cancer and arthritis diagnostics and therapy.

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