作者:Jillian J. Goetz1, Jeffrey M. Trimarchi1
1Department of Genetics, Development and Cell Biology, Neuroscience Program,Iowa State University
简介:A method for the isolation of single retinal cells and subsequent amplification of their cDNAs is described. Single-cell transcriptomics reveals the degree of cellular heterogeneity present in a tissue and uncovers new marker genes for rare cell populations. The accompanying protocol can be adjusted to suit many different cell types.