Surgical Technique for the Implantation of Tissue Engineered Vascular Grafts and Subsequent In Vivo Monitoring

作者： Maxwell T. Koobatian1, Carmon Koenigsknecht2, Sindhu Row3, Stelios Andreadis3, Daniel Swartz2 1Department of Physiology & Bio-Physics,State University of New York Buffalo School of Medicine, 2Department of Pediatrics,State University of New York Buffalo School of Medicine, 3Department of Chemical and Biological Engineering,State University of New York Buffalo School of Engineering

简介：

Overview

This protocol outlines the surgical implantation of tissue engineered vessels (TEVs) into the carotid artery of sheep. It includes real-time digital assessment of blood flow and arterial pressures during the procedure.

Key Study Components

Area of Science

• Vascular surgery
• Tissue engineering
• In vivo assessment

Background

• Importance of vascular grafts in surgical procedures.
• Challenges in achieving successful anastomosis.
• Need for real-time monitoring of vascular graft performance.
• Use of sheep as a model for human vascular studies.

Purpose of Study

• To provide a reproducible protocol for TEV implantation.
• To assess the effectiveness of TEVs in vivo.
• To monitor hemodynamic changes post-implantation.

Methods Used

• Excising a section of the native carotid artery.
• Suturing the TEV using simple interrupted stitches.
• Establishing anastomosis between the graft and artery.
• Using flow probe catheters for real-time monitoring.

Main Results

• Successful implantation of TEVs with stable anastomosis.
• Real-time data on blood flow and arterial pressures collected.
• Demonstrated feasibility of the protocol for future studies.
• Potential for application in human vascular surgeries.

Conclusions

• The protocol provides a reliable method for TEV implantation.
• Real-time monitoring enhances understanding of graft performance.
• Findings support further research into vascular graft applications.

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