Voltage and Calcium Dual Channel Optical Mapping of Cultured HL-1 Atrial Myocyte Monolayer

作者： Jiajie Yan*1, Justin K. Thomson*1, Weiwei Zhao1, Vladimir G. Fast2, Tong Ye3, Xun Ai1 1Department of Cell and Molecular Physiology,Loyola University Chicago, 2Department of Biomedical Engineering,University of Alabama at Birmingham, 3Department of Bioengineering,Clemson University

简介：

Overview

This article describes a technique for dual channel optical mapping in cultured HL-1 atrial cell monolayers. The protocol enables simultaneous visualization of calcium and voltage activity, facilitating detailed analysis of electrophysiological properties.

Key Study Components

Area of Science

• Electrophysiology
• Cell Culture
• Fluorescence Microscopy

Background

• HL-1 atrial cells are a model for studying cardiac electrophysiology.
• Understanding calcium and voltage dynamics is crucial for cardiac research.
• Simultaneous mapping provides insights into cellular behavior.
• This technique enhances the analysis of action potentials and calcium transients.

Purpose of Study

• To map transmembrane potentials and calcium transients in HL-1 monolayers.
• To visualize the propagation of electrical signals across cell layers.
• To improve understanding of cardiac cell interactions.

Methods Used

• Culturing HL-1 cells on a cover slip to achieve proper density.
• Loading cells with calcium and voltage-sensitive dyes.
• Stimulating the monolayer with a bipolar electrode.
• Recording action potentials and calcium transients using fluorescence microscopy.

Main Results

• Successful simultaneous mapping of calcium and voltage activity.
• Clear visualization of signal propagation across the monolayer.
• Detailed traces of action potentials and calcium transients obtained.
• Insights into the electrophysiological properties of HL-1 cells.

Conclusions

• The dual channel optical mapping technique is effective for cardiac research.
• This method enhances the understanding of cellular electrophysiology.
• Future studies can build on these findings to explore cardiac function.

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