Human Vastus Lateralis Skeletal Muscle Biopsy Using the Weil-Blakesley Conchotome

作者： Alicja M. Baczynska1,2, Sarah Shaw3, Helen C. Roberts1,2,3,5, Cyrus Cooper2,3,4, Avan Aihie Sayer1,2,3,5,6, Harnish P. Patel1,2,3 1Academic Geriatric Medicine,University of Southampton, University Hospital Southampton, 2National Institute for Health Research Southampton Biomedical Research Center,University of Southampton and University Hospital Southampton NHS Foundation Trust, 3MRC Lifecourse Epidemiology Unit,University of Southampton, 4National Institute for Health Research Musculoskeletal Biomedical Research Unit,University of Oxford, 5National Institute for Health Research Collaboration for Leadership in Applied Health Research and Care, 6Newcastle University Institute of Ageing and Institute of Health and Society,Newcastle University

简介：

Overview

This video demonstrates a safe and effective technique for percutaneous muscle biopsy of the vastus lateralis. The method aims to obtain sufficient tissue for histochemistry and molecular analysis with minimal discomfort.

Key Study Components

Area of Science

• Neuroscience
• Biology

Background

• Muscle biopsy is important for diagnostic and research studies.
• Obtaining muscle tissue can be challenging due to discomfort and trauma.
• This technique aims to minimize these issues.
• It is applicable in both clinical and research settings.

Purpose of Study

• To introduce a well-tolerated muscle biopsy technique.
• To ensure sufficient tissue is collected for analysis.
• To reduce participant discomfort during the procedure.

Methods Used

• Participants lie in a supine position.
• The thigh is exposed and prepared for the procedure.
• A disposable absorbent sheet is placed under the thigh.
• The Weil-Blakesley conchotome is utilized for the biopsy.

Main Results

• The technique is safe with low complication rates.
• Discomfort is minimized when performed correctly.
• Sufficient muscle tissue is obtained for analysis.

Conclusions

• This method is effective for muscle biopsies.
• It is suitable for both clinical and research applications.
• Participants experience minimal discomfort.

Frequently Asked Questions