Intraductal Injection of LPS as a Mouse Model of Mastitis: Signaling Visualized via an NF-κB Reporter Transgenic

作者：Whitney Barham1, Taylor Sherrill2, Linda Connelly3, Timothy S. Blackwell2, Fiona E. Yull1 1Cancer Biology Department,Vanderbilt University Medical Center, 2Department of Medicine,Vanderbilt University Medical Center, 3Department of Pharmaceutical Sciences,University of Hawaii at Hilo College of Pharmacy

简介：Described here is a technique in which lipopolysaccharide is injected into the lactating mouse mammary gland via the nipple to simulate mastitis, a condition commonly caused by bacterial infection. Lipopolysaccharide injection results in increased nuclear factor kappa B (NF-κB) signaling, visualized through bioluminescent imaging of an NF-κB luciferase reporter mouse.

标签: Intraductal Injection, LPS, Mouse Model, Mastitis, NF-ÎºB Reporter, Transgenic Animal, Disease Progression, Pre-clinical Models, Lipopolysaccharide, Lactating Mouse Mammary Gland, Simulated Infection, Inflammation, Nuclear Factor Kappa B, Bioluminescent Imaging, Luciferase Reporter Mouse, Redness, Swelling, Immune Cell Infiltration, Straight-forward Method, Insulin Syringe, Mammary Duct,

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