Laparoscopic Technique for Serial Collection of Liver and Mesenteric Lymph Nodes in Macaques

作者： Alexander S. Zevin1, Cassie Moats2, Drew May2, Solomon Wangari2, Charlene Miller1, Joel Ahrens2, Naoto Iwayama2, Megan Brown2, Debbie Bratt2, Nichole R. Klatt1, Jeremy Smedley2 1Department of Pharmaceutics,Washington National Primate Research Center, University of Washington, 2Division of Primate Resources,Washington National Primate Research Center, University of Washington

简介：

Overview

This article describes a minimally invasive laparoscopic technique for serial sampling of liver and mesenteric lymph nodes in macaques. This method enhances sampling frequency while minimizing surgical complications compared to traditional laparotomy.

Key Study Components

Area of Science

• Neuroscience
• Microbial translocation
• HIV biology

Background

• Microbial translocation from the GI tract can impact immunological responses.
• Understanding the effects on mesenteric lymph nodes and liver is crucial for HIV research.
• Traditional surgical methods may pose higher risks to animal welfare.
• This technique aims to improve the frequency of sampling with reduced impact on the subjects.

Purpose of Study

• To enhance the assessment of microbial translocation effects.
• To improve animal welfare during research procedures.
• To provide critical samples for understanding HIV pathology.

Methods Used

• Preparation of the macaque for laparoscopic surgery.
• Creation of pneumoperitoneum for abdominal access.
• Collection of mesenteric lymph node and liver biopsies.
• Use of sterile techniques to minimize infection risk.

Main Results

• The technique allows for multiple sampling points with minimal animal distress.
• Successful collection of lymph node and liver biopsies was achieved.
• Improved understanding of the immunological consequences of HIV infection.

Conclusions

• This laparoscopic method is effective for studying microbial translocation.
• It offers a safer alternative to traditional surgical methods.
• The technique can be adapted for other research models involving GI inflammation.

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