A Genetically Engineered Mouse Model of Sporadic Colorectal Cancer

作者： Alexander M. Betzler1, Susan Kochall1, Linda Blickensdörfer2, Sebastian A. Garcia1, May-Linn Thepkaysone1, Lahiri K. Nanduri1, Michael H. Muders3, Jürgen Weitz1,4,5, Christoph Reissfelder1, Sebastian Schölch1,4,5 1Department of Gastrointestinal, Thoracic and Vascular Surgery, Medizinische Fakultät Carl Gustav Carus,Technische Universität Dresden, 2Department of General, Gastrointestinal and Transplant Surgery,University of Heidelberg, 3Department of Pathology, Medizinische Fakultät Carl Gustav Carus,Technische Universität Dresden, 4German Cancer Consortium (DKTK), 5German Cancer Research Center (DKFZ)

简介：

Overview

This article presents a protocol for establishing a genetically engineered mouse model of colorectal cancer through segmental adeno-cre infection. The model is monitored using high-resolution colonoscopy, providing a valuable tool for studying colorectal cancer biology and treatment.

Key Study Components

Area of Science

• Colorectal cancer research
• Genetically engineered mouse models
• Oncology

Background

• Colorectal cancer (CRC) is a significant health concern.
• Genetically engineered mouse models are essential for cancer research.
• Segmental adeno-cre infection allows for localized tumor development.
• High-resolution colonoscopy aids in tumor surveillance.

Purpose of Study

• To establish a reproducible model for CRC research.
• To facilitate the study of CRC biology and treatment options.
• To provide a method accessible to labs with small rodent surgery experience.

Methods Used

• Segmental adeno-cre infection of the colon.
• Use of anesthetized mice for surgical procedures.
• Application of high-resolution colonoscopy for tumor monitoring.
• Creation of colon-localized tumors for research purposes.

Main Results

• The protocol allows for the successful establishment of localized tumors.
• High reproducibility of results across different trials.
• Accessibility for labs with basic surgical skills.
• Potential to answer critical questions in CRC research.

Conclusions

• The established model is a valuable tool for CRC studies.
• It enhances understanding of CRC biology and treatment.
• Future research can build on this methodology for further insights.

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