简介：
Overview
This protocol outlines a comprehensive kidney work-up for mouse models of glomerular disease, facilitating in-depth functional, structural, and mechanistic analyses of glomerular function.
Key Study Components
Area of Science
- Nephrology
- Glomerular Disease
- Mouse Models
Background
- Understanding glomerular disease is crucial for developing therapeutic strategies.
- Mouse models provide a valuable platform for studying kidney pathology.
- This protocol allows for detailed assessments of glomerular function.
- Adaptable to various models of glomerular disease.
Purpose of Study
- To evaluate the functional and structural phenotype of glomeruli.
- To conduct mechanistic analyses of kidney pathology.
- To provide a standardized method for assessing glomerular health.
Methods Used
- Urinary albumin creatinine ratio assessment in metabolic cages.
- Histological analysis using paraformaldehyde fixation and paraffin embedding.
- Isolation of glomeruli through sieving techniques.
- Electron microscopy for detailed structural examination.
Main Results
- VEGF-A knockout mice exhibit increased glomerular permeability.
- VEGF165b overexpression protects against albuminuria.
- Electron microscopy reveals structural abnormalities in knockout mice.
- Periodic acid Schiff staining shows no glomerular abnormalities in light microscopy.
Conclusions
- This protocol enables comprehensive analysis of glomerular disease in mice.
- Findings highlight the role of VEGF in glomerular function.
- Methodology can be applied to various models for broader insights.
What is the main focus of this protocol?
The protocol focuses on conducting a full kidney work-up in mouse models of glomerular disease.
How does this method contribute to glomerular disease research?
It allows for detailed functional, structural, and mechanistic analyses of glomerular function.
What are the advantages of using mouse models?
Mouse models are adaptable and provide insights into the pathology of glomerular diseases.
What techniques are used for glomeruli isolation?
The protocol utilizes sieving techniques with ascending micron pore sizes for glomeruli isolation.
What are the key findings regarding VEGF-A knockout mice?
VEGF-A knockout mice show increased glomerular permeability and structural changes.
How is histological analysis performed in this study?
Histological analysis is performed using paraformaldehyde fixation followed by paraffin embedding.
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