Generating a Murine Orthotopic Metastatic Breast Cancer Model and Performing Murine Radical Mastectomy

作者： Eriko Katsuta1, Masanori Oshi1, Omar M. Rashid2,3,4,5, Kazuaki Takabe1,6,7,8,9,10 1Breast Surgery, Department of Surgical Oncology,Roswell Park Comprehensive Cancer Center, 2Holy Cross Hospital Michael and Dianne Bienes Comprehensive Cancer Center, 3Department of Surgery,Massachusetts General Hospital, 4Department of Surgery,University of Miami Miller School of Medicine, 5Department of Surgery,Nova Southeastern University School of Medicine, 6Department of Surgery,University at Buffalo Jacobs School of Medicine and Biomedical Sciences, 7Department of Breast Surgery and Oncology,Tokyo Medical University, 8Department of Surgery,Yokohama City University, 9Department of Surgery,Niigata University Graduate School of Medical and Dental Sciences, 10Department of Surgery,Fukushima Medical University

简介：

Overview

This article introduces a murine orthotopic breast cancer model utilizing bioluminescence technology to quantify tumor burden. This model aims to closely mimic human breast cancer progression and treatment.

Key Study Components

Area of Science

• Breast cancer research
• Mouse models
• Bioluminescence imaging

Background

• The study addresses key questions in breast cancer progression.
• It highlights the advantages of a high tumorigenesis rate in the model.
• The model also shows a low local recurrence rate post-mastectomy.
• Understanding these factors is crucial for developing effective treatments.

Purpose of Study

• To create a reliable mouse model for studying breast cancer.
• To evaluate tumor burden using advanced imaging techniques.
• To facilitate research on human-like breast cancer progression.

Methods Used

• Confirming lack of response to toe pinch in anesthetized mice.
• Surgical preparation including sterilization of the area.
• Making a five-millimeter skin incision on the chest wall.
• Using microdissection scissors for skin detachment.

Main Results

• The model demonstrates a high rate of tumor development.
• Low recurrence rates after surgical intervention were observed.
• Bioluminescence effectively quantifies tumor burden.
• The model provides insights into human breast cancer dynamics.

Conclusions

• This murine model is a valuable tool for breast cancer research.
• It allows for the investigation of treatment responses.
• The findings can contribute to better understanding of cancer progression.

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