Lung Fixation under Constant Pressure for Evaluation of Emphysema in Mice

作者： Keiko Karasutani1,2, Hario Baskoro1, Tadashi Sato1, Naoko Arano1, Yohei Suzuki1, Aki Mitsui1, Naoko Shimada1, Yuzo Kodama1, Kuniaki Seyama1, Yoshinosuke Fukuchi1, Kazuhisa Takahashi1 1Department of Respiratory Medicine, Juntendo University Graduate School of Medicine,Juntendo University, 2Pharmaceutical Planning Group,Otsuka Pharmaceutical Co., Ltd.

简介：

Overview

This protocol outlines a method for lung fixation that ensures stable conditions for histological evaluation of lung specimens from a mouse model of emphysema. The technique allows for the fixation of multiple lungs simultaneously without collapse or deflation.

Key Study Components

Area of Science

• Neuroscience
• Biology
• Histology

Background

• Emphysema is a lung condition often induced by cigarette smoke.
• Histological evaluation is crucial for understanding lung pathology.
• Maintaining lung inflation during fixation is essential for accurate assessment.
• This protocol addresses challenges in lung fixation techniques.

Purpose of Study

• To present a reliable method for lung fixation.
• To facilitate the evaluation of lung specimens in research.
• To improve consistency in histological analysis of emphysema models.

Methods Used

• Use of a fixation device that maintains constant pressure.
• Incision along the medial line of the mouse to access the thoracic cavity.
• Puncturing the diaphragm to open the thoracic space.
• Visualization of lungs and heart for specimen collection.

Main Results

• Successful maintenance of lung inflation during fixation.
• Ability to fix multiple lungs simultaneously.
• Stable histological specimens suitable for evaluation.
• Demonstration of the procedure by a graduate student.

Conclusions

• The protocol provides a reliable method for lung fixation.
• It enhances the quality of histological evaluations in research.
• This technique can be applied to various studies involving lung pathology.

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