A Pre-Clinical Porcine Model of Orthotopic Heart Transplantation

作者： Roberto V. P. Ribeiro1,2, Juglans S. Alvarez1, Frank Yu1, Mitchell B. Adamson1,2, Naoto Fukunaga1, Cyril Serrick3, Ved Bissoondath1, Massimiliano Meineri4,5, Mitesh V. Badiwala1,6, Vivek Rao1,2,6 1Division of Cardiovascular Surgery, Peter Munk Cardiac Center, Toronto General Hospital,University Health Network, 2Institute of Medical Science,University of Toronto, 3Perfusion and Anesthesia Services, Toronto General Hospital,University Health Network, 4Department of Anesthesia and Pain Management, Toronto General Hospital,University Health Network, 5Department of Anesthesia,University of Toronto, 6Department of Surgery, Faculty of Medicine,University of Toronto

简介：

Overview

This article describes a pre-clinical large-animal (porcine) model of orthotopic heart transplantation. This model has been established to investigate novel cardioprotective strategies.

Key Study Components

Area of Science

• Cardiac surgery
• Heart transplantation
• Cardioprotection

Background

• The porcine heart model is similar to the human heart in anatomy and physiology.
• Using large-animal models is crucial for reliable clinical translation of heart preservation strategies.
• This model allows for the evaluation of new heart preservation techniques.
• Porcine hearts provide an almost ideal platform for research due to their size and functional similarities to human hearts.

Purpose of Study

• To evaluate novel cardioprotective strategies in heart transplantation.
• To establish a reliable pre-clinical model for future clinical applications.
• To enhance understanding of heart preservation methods.

Methods Used

• Preparation of the donor pig by anesthetizing and opening the pericardium.
• Careful retraction of the right ventricular outflow tract and pulmonary artery.
• Dissection of the aortal pulmonary space using cautery.
• Placement of a purse string stitch on the proximal ascending aorta for cannula insertion.

Main Results

• The established model allows for effective investigation of heart preservation techniques.
• Demonstrated the feasibility of using porcine hearts for cardioprotective strategy evaluation.
• Provided insights into surgical techniques relevant to heart transplantation.
• Facilitated the development of methods for improving heart preservation.

Conclusions

• The porcine model is a valuable tool for cardiac research.
• It supports the translation of findings to clinical settings.
• Future studies can build on this model to enhance heart transplantation outcomes.

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