简介：
Overview
This article describes a surgical protocol for inducing robust descending thoracic aortic aneurysms in mice. The method involves left thoracotomy and the application of porcine pancreatic elastase to the aortic wall.
Key Study Components
Area of Science
- Neuroscience
- Cardiovascular research
- Animal models
Background
- Thoracic aortic aneurysms are significant cardiovascular conditions.
- Existing models may lack consistency in aneurysm size and location.
- Reliable protocols are essential for studying aneurysm development.
- This study aims to provide a straightforward surgical approach.
Purpose of Study
- To establish a consistent method for inducing thoracic aortic aneurysms in mice.
- To improve the reliability of animal models for cardiovascular research.
- To facilitate safe access to the murine chest during surgical procedures.
Methods Used
- Induction of anesthesia in male C57BL/6 mice.
- Positioning of the mouse for optimal surgical access.
- Left thoracotomy to expose the thoracic aorta.
- Application of a sponge soaked in porcine pancreatic elastase to the aortic wall.
Main Results
- The protocol consistently produces robust thoracic aortic aneurysms.
- Aneurysms are of predictable size and location.
- The method is straightforward and efficient compared to other models.
- Safe access to the murine chest is achieved through this protocol.
Conclusions
- This surgical protocol is effective for inducing thoracic aortic aneurysms in mice.
- It provides a reliable model for future cardiovascular studies.
- Further research can build upon this method to explore aneurysm pathophysiology.
What is the significance of this protocol?
It provides a reliable method for studying thoracic aortic aneurysms in mice, which is crucial for cardiovascular research.
How does this method compare to other models?
This protocol is more straightforward and produces consistent results in terms of aneurysm size and location.
What type of anesthesia is used?
An appropriate level of sedation is confirmed before the procedure, ensuring the mouse is safely anesthetized.
What are the key steps in the surgical procedure?
Key steps include left thoracotomy, exposure of the thoracic aorta, and application of elastase.
What age and strain of mice are used?
The protocol is designed for eight to ten-week-old male C57BL/6 mice.
Is this protocol safe for the animals?
Yes, the protocol is designed to ensure safe access to the murine chest during the procedure.
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