Isolation and Cultivation of Mandibular Bone Marrow Mesenchymal Stem Cells in Rats

作者： Yueyang Hong*1, Hongyuan Xu*1, Yiling Yang1, Siru Zhou1, Anting Jin1, Xiangru Huang1, Qinggang Dai2, Lingyong Jiang1 1Center of Craniofacial Orthodontics, Department of Oral and Cranio-maxillofacial Science, Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology,National Clinical Research center of Stomatology, 2The 2nd Dental Center, Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology,National Clinical Research center of Stomatology

简介：

Overview

This article presents a method that combines whole bone marrow adherence and flow cytometry sorting for isolating, cultivating, sorting, and identifying bone marrow mesenchymal stem cells from rat mandibles. Mandibular BMSCs are considered a preferred resource for developing therapeutics for craniofacial diseases.

Key Study Components

Area of Science

• Neuroscience
• Stem Cell Research
• Craniofacial Biology

Background

• BMSCs from craniofacial bones show superior capabilities compared to those from other bones.
• Understanding the anatomy and movement of the rat mandible is crucial for this protocol.
• The method aims to obtain significant numbers of purified mandibular BMSCs efficiently.
• Related literature and videos can enhance comprehension of the procedure.

Purpose of Study

• To isolate and cultivate bone marrow mesenchymal stem cells from rat mandibles.
• To provide a reliable protocol for researchers in craniofacial disease therapeutics.
• To highlight the anatomical considerations necessary for successful isolation.

Methods Used

• Whole bone marrow adherence technique.
• Flow cytometry sorting for cell purification.
• Preparation of rat mandibles post-euthanasia.
• Incision techniques for accessing the mandible.

Main Results

• Successful isolation of significant numbers of mandibular BMSCs.
• Demonstration of the effectiveness of the combined method.
• Insights into the anatomical structure aiding the procedure.
• Potential applications in craniofacial disease therapies.

Conclusions

• The protocol provides an efficient method for isolating BMSCs.
• Understanding the anatomy is critical for successful application.
• Mandibular BMSCs hold promise for future therapeutic developments.

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