Craniotomy Procedure for Visualizing Neuronal Activities in Hippocampus of Behaving Mice

作者： YangDong Wang1,2,3,4, DanYang Zhu5, BaoYue Liu1,2,3,6, Kiryl D. Piatkevich1,2,3 1School of Life Sciences,Westlake University, 2Westlake Laboratory of Life Sciences and Biomedicine, 3Institute of Basic Medical Sciences,Westlake Institute for Advanced Study, 4School of Basic Medical Sciences,Xi'an JiaoTong University, 5Office of Public Affairs,Westlake University, 6School of Pharmaceutical Sciences,Jilin University

简介：

Overview

This article demonstrates the preparation and implantation of a custom-made imaging window with an infusion cannula on the CA1 region of the hippocampus in mice for in vivo imaging of neuronal activities.

Key Study Components

Area of Science

• Neuroscience
• Neuroengineering
• In vivo Imaging

Background

• The CA1 region of the hippocampus plays a crucial role in memory and learning.
• Neuronal activity in this area is essential for investigating various neurological conditions.
• Existing methods may not allow precise imaging and manipulation of neuronal circuits.
• A custom imaging system can facilitate real-time studies of hippocampal functions.

Purpose of Study

• To demonstrate a detailed surgical procedure for creating an imaging setup in the mouse brain.
• To improve upon existing methods for in vivo imaging of neuronal activities.
• To enable researchers to explore neuronal dynamics in the hippocampus more effectively.

Methods Used

• The study utilizes a custom imaging window design and surgical implantation in mice.
• Mouse models were used to investigate neuronal activities in the hippocampus.
• The implantation procedure involves anesthetic administration, craniotomy, and careful placement of the cannula and window.
• Meticulous surgical steps are tracked to ensure precision and prevent complications.
• Key methodologies include the use of UV curing adhesives and multiple surgical tools for accurate targeting.

Main Results

• The article outlines a successful methodology for careful implantation of an imaging setup.
• Key steps detail how to avoid complications and ensure optimal placement of the cannula.
• The process demonstrates important mechanical insights into maintaining brain viability during implantation.
• The findings highlight the potential for improved in vivo studies of neuronal dynamics in the hippocampus.

Conclusions

• This study illustrates a reproducible method for imaging neuronal activities in mice.
• The procedure enhances understanding of hippocampal functions, with potential implications for neurological research.
• Insights gained from this approach can contribute to the study of mechanisms underlying learning and memory.

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