Rapid Antimicrobial Susceptibility Testing by Stimulated Raman Scattering Imaging of Deuterium Incorporation in a Single Bacterium

作者： Meng Zhang1,2, Mohamed N. Seleem3, Ji-Xin Cheng1,2,4,5 1Department of Electrical and Computer Engineering,Boston University, 2Boston University Photonics Center,Boston University, 3Department of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary Medicine,Virginia Polytechnic Institute and State University, 4Department of Biomedical Engineering,Boston University, 5Department of Chemistry,Boston University

简介：

Overview

This study describes a rapid antimicrobial susceptibility testing (AST) assay that can be completed in 2.5 hours using single-cell-stimulated Raman scattering imaging of D2O metabolism. This protocol is significant as it allows for testing of bacterial samples from urine and whole blood, providing a transformative approach to rapid single-cell phenotypic AST in clinical settings.

Key Study Components

Research Area

• Antimicrobial susceptibility testing
• Clinical microbiology
• Rapid diagnostic methods

Background

• Conventional AST methods require longer processing times.
• Rapid methods could lead to timely and targeted treatments for patients.
• Single-cell analysis provides detailed insights into bacterial behavior.

Methods Used

• Single-cell-stimulated Raman scattering (SRS) imaging
• Bacteria from urine and whole blood
• Sequential dilution and antibiotic treatment of bacterial samples

Main Results

• Successful implementation of rapid AST within 2.5 hours.
• Effectiveness demonstrated in monitoring bacterial metabolic activity.
• Conclusions aligned with the potential for clinical application.

Conclusions

• This study demonstrates a rapid and effective method for AST using SRS imaging.
• The approach has the potential to significantly improve the speed and accuracy of bacterial diagnostics in clinical microbiology.

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