A Porcine Heterotopic Heart Transplantation Protocol for Delivery of Therapeutics to a Cardiac Allograft

作者： Michelle Mendiola Pla1, Amy Evans2, Franklin H. Lee3, Yuting Chiang1, Muath Bishawi1, Andrew Vekstein1, Lillian Kang1, Diego Zapata4, Ryan Gross3, Alexis Carnes2, Lynden E. Gault5, Julie A. Balko6, Desiree Bonadonna2, Sam Ho5, Paul Lezberg7, Benjamin S. Bryner1, Jacob N. Schroder1, Carmelo A. Milano1, Dawn E. Bowles3 1Division of Cardiothoracic Surgery, Department of Surgery,Duke University, 2Perfusion Services,Duke University, 3Division of Surgical Sciences, Department of Surgery,Duke University, 4Division of Laboratory Animal Resources,Duke University, 5Gift of Hope Organ and Tissue Donor Network, 6College of Veterinary Medicine,North Carolina State University, 7TransMedics, Inc

简介：

Overview

This protocol describes the administration of therapeutics to a whole cardiac allograft in a porcine heterotopic heart transplant model. It allows for extended testing of therapeutic effects on cardiac allografts post-transplantation.

Key Study Components

Area of Science

• Cardiac transplantation
• Therapeutic delivery
• Ex vivo perfusion techniques

Background

• This is the first protocol for administering therapeutics to an entire cardiac allograft.
• The technique is designed for large animal models.
• It addresses challenges in optimizing exposure during transplantation.
• Experienced perfusionists are essential for monitoring the procedure.

Purpose of Study

• To investigate interventions that prevent cardiac allograft rejection.
• To minimize postoperative immunosuppression.
• To extend the lifetime of cardiac allografts.

Methods Used

• Normothermic ex vivo sanguinous perfusion system.
• Large animal model of cardiac transplantation.
• Incision technique for access to the heart.
• Coordination among multiple team members for procedure execution.

Main Results

• Effective administration of therapeutics to cardiac allografts.
• Ability to test therapeutic effects over extended periods.
• Potential to improve outcomes in cardiac transplantation.

Conclusions

• This protocol enhances the understanding of cardiac allograft management.
• It provides a framework for future research on cardiac transplantation.
• Further studies can build on this method to improve transplant success.

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