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Overview
This article demonstrates a surgical procedure for delivering embryonic stem cell-derived endothelial cells to the ischemic hindlimb. The cells are tracked non-invasively using bioluminescence imaging to assess their therapeutic effects.
Key Study Components
Area of Science
- Neuroscience
- Stem Cell Research
- Cardiovascular Medicine
Background
- Peripheral arterial disease (PAD) causes narrowing of peripheral arteries.
- PAD leads to reduced blood flow and oxygen supply to the legs and feet.
- Stem cells have potential therapeutic effects for treating PAD.
- Bioluminescence imaging allows tracking of stem cells in vivo.
Purpose of Study
- To investigate the use of embryonic stem cell-derived endothelial cells in treating PAD.
- To determine the homing ability of these cells to the site of ischemia.
- To evaluate the therapeutic effects of the injected cells.
Methods Used
- Induction of ischemia in the hind limb.
- Injection of embryonic stem cell-derived endothelial cells.
- Modification of cells to express firefly luciferase for tracking.
- Non-invasive bioluminescence imaging to monitor cell behavior.
Main Results
- Successful delivery of stem cells to the ischemic hindlimb.
- Cells were tracked effectively using bioluminescence imaging.
- Evidence of cell homing to the site of injury.
- Potential therapeutic effects observed in the treated area.
Conclusions
- Embryonic stem cell-derived endothelial cells can be effectively delivered to ischemic tissues.
- Bioluminescence imaging is a valuable tool for tracking stem cell therapies.
- This approach may offer new avenues for treating peripheral arterial disease.
What is peripheral arterial disease?
Peripheral arterial disease (PAD) is a condition characterized by the narrowing of peripheral arteries, leading to reduced blood flow to the limbs.
How are stem cells used in this study?
Embryonic stem cell-derived endothelial cells are injected into the hind limb to assess their therapeutic potential for treating PAD.
What imaging technique is used in this research?
Bioluminescence imaging is used to non-invasively track the stem cells after injection.
What is the significance of using firefly luciferase?
Firefly luciferase allows for the tracking of stem cells in vivo, enabling researchers to monitor their location and behavior.
What are the potential outcomes of this study?
The study aims to demonstrate the effectiveness of stem cell therapy in improving blood flow and healing in ischemic tissues.
Who conducted this research?
The research was conducted by John Cook, a professor of medicine in the division of Cardiovascular medicine at Stanford University.
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