Clinical Microfluidic Chip Platform for the Isolation of Versatile Circulating Tumor Cells

作者： Hongmei Chen1, Yufeng Han1, Qingli Li2, Yong Zou1, Shuangshou Wang3, Xiaodong Jiao4 1School of Microelectronics and Data Science,Anhui University of Technology, 2Shanghai Key Laboratory of Multidimensional Information Processing,East China Normal University, 3School of Chemistry and Chemical Engineering,Anhui University of Technology, 4Department of Medical Oncology,Changzheng Hospital

简介：

Overview

This protocol describes a method for enumerating red tumor cells and isolating circulating tumor cells (CTCs) using a clinical microfluidic chip. This technique simplifies blood sample preprocessing and allows for rapid detection and identification of single CTCs.

Key Study Components

Area of Science

• Biomedical analysis
• Clinical diagnostics
• Cell culture techniques

Background

• Circulating tumor cells (CTCs) are critical for cancer diagnosis and monitoring.
• Microfluidic chips enhance the efficiency of CTC isolation.
• Immunofluorescent staining aids in the visualization of CTCs.
• Standard cell culture methods are essential for preparing tumor cells for analysis.

Purpose of Study

• To develop a reliable method for CTC enumeration.
• To improve the efficiency of clinical blood sample processing.
• To facilitate the identification of CTCs in situ.

Methods Used

• Culture of tumor cell lines (MCF-7, MDA-MB-231, HeLa) in DMEM.
• Staining of cells with Calcein AM for visualization.
• Detachment of cells using trypsin for enumeration.
• Use of a microfluidic chip for CTC isolation and analysis.

Main Results

• Successful enumeration of red tumor cells.
• Efficient isolation of CTCs from blood samples.
• Demonstration of the microfluidic chip's effectiveness in clinical settings.
• Rapid identification of single CTCs through immunofluorescent staining.

Conclusions

• The microfluidic chip is a valuable tool for CTC analysis.
• This method enhances the speed and accuracy of cancer diagnostics.
• Further studies could expand its applications in clinical oncology.

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