A Bright NIR-II Fluorescence Probe for Vascular and Tumor Imaging

作者： Yang Li*1,2, Xue Qiao*1, Xuechuan Hong1,2 1College of Science, Research Center for Ecology, Laboratory of Extreme Environmental Biological Resources and Adaptive Evolution,Tibet University, 2State Key Laboratory of Virology, Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (MOE) and Hubei Province Engineering and Technology Research Center for Fluorinated Pharmaceuticals,Wuhan University School of Pharmaceutical Sciences

简介：

Overview

This protocol outlines a real-time NIR-II fluorescence imaging operation for mice using a specialized imaging device. It highlights the significance of high-resolution imaging in vascular and tumor studies.

Key Study Components

Area of Science

• Neuroscience
• Biomedical Imaging
• Fluorescence Imaging

Background

• NIR-II imaging allows for deeper visualization in living subjects.
• It is effective for studying vascular diseases and cancer.
• This technique enhances opportunities for biomedical research.
• Commercially available materials are used to prepare for imaging.

Purpose of Study

• To demonstrate the use of NIR-II fluorescence imaging in live mice.
• To provide a detailed protocol for researchers.
• To improve imaging techniques for vascular and tumor analysis.

Methods Used

• Preparation of the imaging setup with black cardboard.
• Placement of the sample for optimal imaging.
• Selection of appropriate filters for imaging.
• Adjustment of platform height using touchscreen controls.

Main Results

• Successful high-resolution imaging of vascular structures.
• Effective visualization of tumor environments.
• Demonstration of the imaging device's capabilities.
• Potential applications in clinical settings.

Conclusions

• NIR-II fluorescence imaging is a valuable tool for biomedical research.
• It provides insights into vascular diseases and cancer.
• The protocol can be utilized by researchers for various applications.

Frequently Asked Questions