In Vitro and In Vivo Evaluation of Photocontrolled Biologically Active Compounds – Potential Drug Candidates for Cancer Photopharmacology

作者： Kateryna Horbatok*1,2, Tetyana Makhnii*2, Viktoriia Kosach2, Volodymyr Danko1, Andrey Kovalenko1, Stanislav Fatiushchenkov1, Petro Borysko2, Iryna Pishel2, Oleg Babii3,4, Anne S. Ulrich3, Tim Schober4, Sergii Afonin3, Igor V. Komarov1,2,4 1Taras Shevchenko National University of Kyiv, 2Bienta/Enamine, 3Karlsruhe Institute of Technology, 4Lumobiotics

简介：

Overview

This protocol outlines experiments for evaluating photoswitchable anticancer peptides, focusing on cytotoxicity in 2D and 3D cultures, photoisomerization efficiency, and in vivo efficacy.

Key Study Components

Area of Science

• Biochemistry
• Pharmacology
• Cell Biology

Background

• Photoswitchable compounds can toggle between inactive and active forms using light.
• These compounds aim to enhance drug safety by targeting specific areas in the body.
• Standard protocols for developing photopharmacology drugs are lacking.
• This study presents methods for early preclinical research on these compounds.

Purpose of Study

• To evaluate the efficacy of photoswitchable anticancer peptides.
• To establish protocols for cytotoxicity assessment in various cell culture models.
• To demonstrate the procedures for assessing photoisomerization efficiency.

Methods Used

• Preparation of stock solutions of peptides and controls.
• Cytotoxicity assays in 2D and 3D cell cultures.
• Photoisomerization of compounds using specific light wavelengths.
• Automated fluorescence imaging for cell viability analysis.

Main Results

• Demonstrated effective cytotoxicity of photoswitchable peptides in both culture models.
• Established protocols for measuring photoisomerization efficiency.
• Identified optimal conditions for in vivo efficacy assessments.

Conclusions

• Photoswitchable peptides show promise as safer anticancer agents.
• The developed protocols can facilitate further research in photopharmacology.
• Future studies should focus on clinical applications of these findings.

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