Generation of Functional Endodermal Hepatic Organoids

作者： Soheil Akbari*1, Nevin Ersoy*1,2,3, Alper Bagriyanik1,2,4, Nur Arslan1,5, Tamer Tevfik Önder6, Esra Erdal1,7 1Izmir Biomedicine and Genome Center, 2Department of Histology and Embryology, Health Science Institute,Dokuz Eylul University, 3Department of Histology and Embryology, Faculty of Medicine,Izmir Tinaztepe University, 4Department of Histology and Embryology, Faculty of Medicine,Dokuz Eylul University, 5Department of Pediatric Gastroenterology and Metabolism, Faculty of Medicine,Dokuz Eylul University, 6Department of Medical Biology, Faculty of Medicine,Koc University, 7Department of Medical Biology, Faculty of Medicine,Dokuz Eylul University

简介：

Overview

This protocol describes the generation of fast and reproducible endodermal hepatic organoids (eHEPOs). With this protocol, eHEPOs can be produced within 2 weeks and expand long-term (more than 1 year) without losing their differentiation and functionality.

Key Study Components

Research Area

• Developmental biology
• Organoid culture
• Liver biology

Background

• Importance of hepatic organoids in study of liver diseases
• Advancements in organoid technology for regenerative medicine
• Need for reproducibility and functionality in organoid models

Methods Used

• Protocol for generating endodermal hepatic organoids
• Long-term culture techniques
• Assessment of differentiation and functionality

Main Results

• Successful generation of eHEPOs in 2 weeks
• Long-term expansion exceeding 1 year
• Maintained differentiation and functionality throughout culture

Conclusions

• The study provides a reliable method for producing eHEPOs.
• This could enhance research in liver biology and regenerative medicine.

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