Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator

作者： Kaede Saito1,2, Yuta Hiraike1,2,3, Misato Oguchi2, Toshimasa Yamauchi2 1Division for Health Service Promotion,The University of Tokyo, 2Department of Diabetes and Metabolic Diseases, Graduate School of Medicine,The University of Tokyo, 3The University of Tokyo Excellent Young Researcher Program,The University of Tokyo

简介：

Overview

This study focuses on the semi-automated isolation of the stromal vascular fraction (SVF) from murine adipose tissue, with the aim of obtaining preadipocytes and promoting adipocyte differentiation in vitro. A novel protocol utilizing a tissue dissociator for collagenase digestion is presented, which enhances reproducibility and reduces experimental variation.

Key Study Components

Research Area

• Adipocyte differentiation
• Energy expenditure in obesity treatments
• Murine models for metabolic research

Background

• Different functions of brown, beige, and white adipocytes
• Need for reproducible methods in adipocyte research
• Identification of critical transcription factors regulating differentiation

Methods Used

• Semi-automated isolation using collagenase digestion
• Murine adipose tissue
• Tissue dissociators and enzymatic assays

Main Results

• Improved methods for isolating SVF and differentiating adipocytes
• Successful differentiation demonstrated via Oil Red O staining and mRNA analysis
• Investigation into the effects of Rosiglitazone on brown fat-specific gene expression

Conclusions

• This protocol facilitates reliable SVF isolation for adipocyte differentiation.
• The findings hold significant implications for obesity and diabetes research.

Frequently Asked Questions