Biobanking of Human Aqueous and Vitreous Liquid Biopsies for Molecular Analyses

作者： Julian Wolf1,2, Teja Chemudupati1,2, Aarushi Kumar1,2, Ditte K. Rasmussen1,2, Karen M. Wai2, Robert T. Chang2, Artis A. Montague2, Peter H. Tang3,4, Alexander G. Bassuk5,6,7, Antoine Dufour8,9, Prithvi Mruthrunjaya2, Vinit B. Mahajan1,2,10 1Molecular Surgery Laboratory,Stanford University, 2Department of Ophthalmology, Byers Eye Institute,Stanford University, 3Department of Ophthalmology and Visual Neurosciences,University of Minnesota, 4Retina Consultants of Minnesota, 5Department of Pediatrics,University of Iowa, 6Department of Neurology,University of Iowa, 7The Iowa Neuroscience Institute (INI),University of Iowa, 8Department of Physiology and Pharmacology, Cumming School of Medicine,University of Calgary, 9Department of Biochemistry and Molecular Biology, Cumming School of Medicine,University of Calgary, 10Veterans Affairs Palo Alto Health Care System

简介：

Overview

This protocol presents an integrated biorepository platform for the standardized collection, annotation, and biobanking of high-quality human aqueous humor and vitreous liquid biopsies for molecular downstream analyses, including proteomics, metabolomics, and glycomics.

Key Study Components

Area of Science

• Ocular diseases
• Liquid biopsies
• Molecular profiling

Background

• Liquid biopsies from the human eye capture locally enriched fluids.
• They contain thousands of different molecules from specialized ocular tissues.
• These biopsies allow for molecular characterization of ocular diseases.
• Potential to identify novel diagnostic and therapeutic strategies.

Purpose of Study

• To develop a protocol for standardized collection and biobanking of aqueous humor and vitreous samples.
• To ensure high-quality samples during intraocular surgery.
• To create a web-based database for sample annotation and tracking.

Methods Used

• Anterior chamber paracentesis using a 30 to 32 gauge needle.
• Manual aspiration of aqueous humor and vitreous samples.
• Use of a barcode system for sample tracking and database entry.
• Transport of samples on dry ice for storage at -80 degrees Celsius.

Main Results

• Successful collection of high-quality aqueous humor and vitreous samples.
• Implementation of a data privacy compliant database for sample annotation.
• Efficient retrieval of samples for downstream analyses.
• Demonstration of a completed sample collection form in REDCap.

Conclusions

• The protocol enables standardized biobanking of ocular liquid biopsies.
• Facilitates molecular analysis for ocular disease research.
• Enhances the potential for novel diagnostic and therapeutic strategies.

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