A Workflow for the Quantitative Assessment of the Endophytic and Epiphytic Bacterial Microbiomes of the Bark of Populus trichocarpa

作者： Tianyu Ma*1,2, Weixiong Wang*1,2, Shuang Li*1,3, Dong Li1,2, Lishi Shen1,3, Guanzheng Qu1,3, Vincent L. Chiang1,4, Qiang Zhang5, Di Wu1,2, Ben Niu1,2 1State Key Laboratory of Tree Genetics and Breeding,Northeast Forestry University, 2College of Life Science,Northeast Forestry University, 3College of Forestry,Northeast Forestry University, 4Forest Biotechnology Group, Department of Forestry and Environmental Resources,North Carolina State University, 5Institute of Economic Forest of Xinjiang Academy of Forestry Sciences

简介：

Overview

This protocol details a comprehensive workflow for assessing the endophytic and epiphytic bacterial microbiomes associated with the bark of Populus trichocarpa. The study emphasizes sample collection, processing, and genomic analysis utilizing next-generation sequencing technology for thorough microbiome profiling.

Key Study Components

Research Area

• Microbiome analysis
• Forestry species
• Bacterial communities

Background

• Study of endophytic and epiphytic bacteria
• Importance of Populus trichocarpa in forestry
• Need for efficient microbiome profiling methods

Methods Used

• Sample collection and processing specific to bark tissues
• Extraction of genomic DNA from endophytic and epiphytic samples
• Next-generation sequencing technology for microbiome analysis

Main Results

• Successful workflow for microbiome profiling
• Clear methodology for genomic DNA extraction and PCR amplification
• Validation of sequencing data obtained from bacterial communities

Conclusions

• This study provides a valuable framework for examining the microbiomes of forestry species.
• The approaches detailed may be applicable to other plant microbiome studies.

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