Establishment of Patient-Derived Xenograft Mouse Model with Human Osteosarcoma Tissues

作者： Xingyuan Sun*1,2, Junli Chang*1,2, Chujie Zhou1,2, Peng Zhao1,2, Suxia Guo1,2, Junjie Tong1,2, Yongjun Wang1,2, Yanping Yang1,2 1Longhua Hospital,Shanghai University of Traditional Chinese Medicine, 2Key Laboratory of theory and therapy of muscles and bones,Ministry of Education

简介：

Overview

This protocol describes the establishment of a patient-derived xenograft (PDX) mouse model using human osteosarcoma tissue. This model allows for the study of osteosarcoma growth, drug resistance, and metastasis.

Key Study Components

Area of Science

• Oncology
• Translational Research
• Preclinical Models

Background

• Osteosarcoma is a common bone cancer in children and adolescents.
• Understanding its biology and treatment response is crucial for improving patient outcomes.
• Patient-derived xenograft models closely mimic human tumors.
• This study focuses on the procedural steps for establishing such models.

Purpose of Study

• To create a reliable PDX model for osteosarcoma research.
• To facilitate the investigation of tumor biology and treatment efficacy.
• To enhance preclinical research methodologies.

Methods Used

• Freshly resected osteosarcoma tissues are collected.
• Tissues are rinsed with sterile physiological saline.
• Areas of bleeding and necrosis are removed.
• The model is established in immunodeficient mice.

Main Results

• The PDX model successfully mimics human osteosarcoma growth.
• It provides insights into drug resistance and metastasis.
• The model demonstrates primary tumor similarities.
• It serves as a platform for future osteosarcoma studies.

Conclusions

• The established PDX model is a valuable tool for osteosarcoma research.
• It aids in understanding the complexities of tumor behavior.
• This model can significantly contribute to the development of new therapies.

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