Infection of In Vivo and In Vitro Pines with the Pinewood Nematode Bursaphelenchus xylophilus and Isolation of Induced Volatiles

作者： Jorge M. S. Faria1,2, A. Cristina Figueiredo3,4, Dora M. Teixeira5, Maria L. Inácio1,2 1INIAV, I.P., National Institute for Agrarian and Veterinary Research, I.P., 2GREEN-IT Bioresources for Sustainability, Instituto de Tecnologia Química e Biológica,Universidade Nova de Lisboa (ITQB NOVA), 3Centre for Environmental and Marine Studies (CESAM Lisboa), Biotecnologia Vegetal (BV), DBV,Faculdade de Ciências da Universidade de Lisboa, 4Centre for Ecology, Evolution and Environmental Changes (CE3C), Biotecnologia Vegetal (BV), DBV,Faculdade de Ciências da Universidade de Lisboa, 5Hercules Laboratory & Chemistry and Biochemistry Department of Science and Technology School,University of Évora

简介：

Overview

This study presents a detailed protocol for investigating the interaction between the pinewood nematode and Pinus pinaster, both in vitro and in vivo. It emphasizes the analysis of volatile organic compounds emitted by infected pine trees, which sheds light on the host's response to these phytoparasites.

Key Study Components

Research Area

• Pinewood nematode infection
• Volatile organic compound analysis
• Plant-pathogen interactions

Background

• The genetic diversity of pine plantlets impacts volatile profiles.
• In vitro co-culture systems provide a controlled environment to reduce variability.
• Understanding host responses is crucial for managing pine wilt disease.

Methods Used

• In vitro co-culture systems of pine shoots and nematodes
• GC and GC-MS for volatile profiling
• Greenhouse bioassays for symptom quantification

Main Results

• The establishment of a 100-fold increase in nematode population within eight days.
• Signs of pine wilt disease observed 20 days post-inoculation.
• Successful isolation of volatiles from the infected tissues.

Conclusions

• This protocol enhances our understanding of plant responses to nematode infection.
• The findings contribute to the broader field of plant pathology and nematology.

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