We describe the procedure to prepare staged Drosophila embryos for the visualization of the embryonic nervous system during embryogenesis.
The overall goal of this procedure is to prepare oph and melanogaster embryos for whole mount visualization of the nervous system. This is accomplished by first collecting embryos from the fly strain of interest. The second step of the procedure is to remove the corium with bleach.
The third step of the procedure is to apply heptane and methanol to remove the vilin membrane. The final step of the procedure is to apply antibodies to the embryos. Ultimately, results can be obtained that show the structure of the drosophila embryo nervous system through immunofluorescence microscopy.
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