Combined Recombinase Polymerase Amplification CRISPR/Cas12a Assay for Detecting Fusarium oxysporum f. sp. cubense Tropical Race 4
作者:
Jos Jansen van Vuuren*1, Megan Ceris Matthews*1, Isabelle Robène1,2, Samuel Rozsasi1,2, Manuela Campa3, Johan Burger3, Altus Viljoen1, Diane Mostert1
1Department of Plant Pathology, Faculty of Agrisciences,Stellenbosch University, 2CIRAD, UMR PVBMT, 3Department of Genetics, Faculty of Agrisciences,Stellenbosch University
简介:
In this protocol, a combined recombinase polymerase amplification CRISPR/Cas12a assay is illustrated for the detection of the invasive plant pathogen, Fusarium oxysporum f. sp. cubense tropical race 4.