A CD4 enumeration method, the α-test, is described which uses whole saliva to provide rapid and accurate CD4 counts. The α-test costs pennies and eliminates the need for technical training, costly reagents such as monoclonal antibodies, instrumentation, refrigeration, transport of samples, as well as collection and handling of blood.
There is an urgent need for affordable CD four enumeration to monitor HIV disease in resource limited regions. This video demonstrates a low cost point of care screening test in which CD four counts are determined based on the presence of the proteinase inhibitor Alpha one PI in saliva. To do this, the subject or patient is first given a lemon drop to stimulate exudation of serous fluid into the saliva.
The saliva is collected in a specimen cup and transferred to a 96 well assay plate. Then a saturating amount of the proteinase porcine pancreatic elastase is added to the saliva where it binds and inhibits alpha one pi. Next colormetric elastase substrate is added.
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