Neointimal hyperplasia is the primary cause of stenosis in arterialized veins. We propose a new protocol whereby the right external jugular vein is grafted using the cuff technique in the common carotid artery of Sprague Dawley rats. The survival rate was 100 % at the time point of sacrifice.
Neointimal hyperplasia is one of the main causes of stenosis in Arterialized veins used in arterial coronary bypass surgery in peripheral arterial bypass surgery, as well as in arterial venous fistulas vein graft. Interposition in the common carotid artery has been performed in several research projects to study the etiology of neointimal hyperplasia and therapeutic options to address it currently. However, conventional microsurgical techniques using small animal models are complicated, frustrating, and require excessive sacrifice of experimental animals to simplify the whole procedure.
This video illustrates a detailed operation protocol, which will help the novice surgeon learn the cut technique and the vein graft. Inter position in the common carted artery 21 female sprig dolly rats from a commercial breeder were categorized in three equal groups that were operated and sacrificed on day 21, 42 and 84 respectively. General anesthesia Of the animals was induced using a plexiglass box connected to an anesthesia machine set at an oxygen flow rate of one liter per minute and an easel fluorine vaporizer with 5%anesthetic gas.
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