Fabrication of a Microfluidic Device for the Compartmentalization of Neuron Soma and Axons

作者：Joseph Harris1, Hyuna Lee1, Behrad Vahidi1, Christina Tu2, David Cribbs3, Noo Li Jeon1, Carl Cotman3 1Department of Biomedical Engineering,University of California, Irvine (UCI), 2Stem Cell Research Center,University of California, Irvine (UCI), 3Institute for Brain Aging and Dementia,University of California, Irvine (UCI)

简介：In this video we demonstrate the technique of soft lithography with polydimethyl siloxane (PDMS) which we use to farbricate a microfluidic device for culturing neurons.

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Overview

This video demonstrates the technique of soft lithography using polydimethylsiloxane (PDMS) to fabricate a microfluidic device for culturing neurons. The device allows for the compartmentalization of neuronal cell bodies and axons, facilitating advanced studies in neurobiology.

Key Study Components

Area of Science

• Neuroscience
• Microfluidics
• Cell Culture Techniques

Background

• The neuron device was first published in Nature Methods in August 2005.
• It has been widely adopted by researchers globally.
• The device is made from PDMS cast on a silicon wafer.
• It features reservoirs and channels designed for neuronal culture.

Purpose of Study

• To demonstrate the fabrication of a microfluidic device for neuron culture.
• To highlight the advantages of using this device over traditional tissue culture platforms.
• To provide a detailed methodology for preparing the neuron device.

Methods Used

• Mixing PDMS with a curing agent in a specific ratio.
• Using a vacuum desiccator to remove air bubbles from the PDMS.
• Curing the PDMS on a hot plate or at room temperature.
• Plasma bonding the PDMS devices to cover slips for enhanced functionality.

Main Results

• The device allows for the compartmentalization of cell bodies and axons.
• It enables separate treatments for different compartments.
• Researchers can obtain pure axonal fractions for study.
• The design facilitates the observation of axonal regeneration.

Conclusions

• The microfluidic neuron device is a valuable tool for neuroscience research.
• It offers significant advantages for studying neuronal behavior and regeneration.
• The fabrication process is detailed and reproducible for researchers.

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