全文：
Overview
This article describes a procedure for creating a mouse model of focal cerebral ischemia by occluding the middle cerebral artery using an intraluminal filament. This model is commonly used to study ischemic stroke and its effects on neurological function.
Key Study Components
Area of Science
- Neuroscience
- Stroke Research
- Animal Models
Background
- Filamentous occlusion of the middle cerebral artery is a widely used method for inducing ischemic stroke in mice.
- The procedure allows for both permanent and transient ischemia.
- It is considered less invasive compared to other techniques, such as craniectomy.
- Behavioral changes in mice can be observed to assess the impact of the occlusion.
Purpose of Study
- To develop a reliable mouse model for studying ischemic stroke.
- To evaluate the effects of different durations of ischemia on neurological deficits.
- To provide a detailed methodology for researchers new to this technique.
Methods Used
- Preparation of an 8.0 nylon filament for occlusion.
- Midline neck incision to access the common carotid artery.
- Use of ligatures and clamps to secure arteries and prevent bleeding.
- Filament insertion into the internal carotid artery to occlude the middle cerebral artery.
Main Results
- Behavioral deficits observed post-occlusion include decreased resistance and circling behavior.
- Histological analysis shows tissue necrosis after 60 minutes of occlusion.
- Shorter occlusion times result in limited neuronal cell death.
- Well-trained surgeons can perform the procedure efficiently.
Conclusions
- The filamentous MCA occlusion technique is effective for studying ischemic stroke.
- Standard operating procedures are crucial for reproducibility.
- Behavioral assessments provide valuable insights into the effects of ischemia.
What is the purpose of the MCA occlusion model?
The MCA occlusion model is used to study ischemic stroke and its neurological effects in mice.
How long does the procedure take?
A well-trained surgeon can perform the MCA occlusion in approximately 10 minutes.
What are the signs of successful occlusion?
Behavioral changes such as circling and decreased resistance indicate successful occlusion.
What type of mice are used for this procedure?
Mice older than 10 weeks are typically used for this model.
How is the filament prepared?
The filament is cut into 11 mm lengths and coated with a silicon mixture for smooth insertion.
What post-operative care is required?
Mice should be monitored for discomfort, provided with mashed food, and checked daily for weight loss.
繁體中文
