This article details the procedure for implanting a telemetry device in mice to measure core body temperature continuously. The method allows for real-time physiological monitoring in freely moving animals.
Telemetry facilitates the continuous real-time measurement of physiological signals via a wireless implanted telemetry device in a freely moving live animal.
To implant a temperature telemetric device, first, prep an anesthetized mouse in the supine position. Next, make a midline incision on the abdominal skin. Separate the skin from the underlying muscle layer.
Now, make a small incision on the linea alba - a thin band of connective tissue that separates the abdominal muscles - to access the intraperitoneal cavity. Through the opened muscle layer, gently transfer the sterile temperature battery-free telemetry device into the intraperitoneal cavity.
Close the surgical incision. The implanted telemetry device floats in the cavity and eliminates excess tension on the abdominal wall. Return the mouse to its cage placed on a telemetry receiver platform.
The telemetry receiver powers the implanted telemetry device. The device continuously measures the mouse's core body temperature and transmits the physiological data through radio waves detected by the receiver.
The receiver transforms this radio signal into a digital signal, which is relayed to the computer-based data acquisition system. The acquisition system converts the signal into the desired format and stores the information for subsequent analysis.
After anesthetizing the mouse, use eye lube, such as the vet ointment, to protect the animal's eyes from damage or injury during surgery. To prepare the surgical site, shave the lower abdomen with small animal hair clippers or a commercially available hair remover. Administer the first dose of subcutaneous 0.1 milligrams per kilogram buprenorphine. Scrub the area with three washes of povidone-iodine or chlorhexidine, followed by 70% isopropyl alcohol rinse.
Then, transfer the mouse to the surgical area. Use an adhesive drape to isolate the surgical site on the mouse. Using sterile instruments and aseptic technique, make an approximately 1-centimeter incision on the midline along the linea alba, about 0.5 centimeters from the costal margin. Then, separate the skin from the muscle layer and make a slightly smaller incision on the linea alba, being careful not to damage the bowels or internal organs.
Once the muscle layer is open, place the sterile telemeter into the intraperitoneal cavity in front of the caudal arteries and veins and dorsal to the digestive organs to allow it to float freely. Close the abdominal opening with a sterile 5-0 absorbable suture and close the skin using a simple interrupted stitch with 5-0 proline suture. Place the mouse in its clean cage with a snuggle microwave heating pad under the cage. Monitor the mouse every 15 minutes during the first hour of recovery from anesthesia and then return to the animal housing facility.
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