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Transcriptome Analysis of Single Cells

作者：Jacqueline Morris*1, Jennifer M. Singh*1, James H. Eberwine1,2 1Department of Pharmacology,University of Pennsylvania, 2The Penn Genome Frontiers Institute,University of Pennsylvania
简介：In this article we describe a simple method for the harvesting of single cells from rat primary neuronal cultures and subsequent transcriptome analysis using aRNA amplification. This approach is generalizable to any cell type.
标签: Transcriptome Analysis,    Single Cells,    Gene Expression Analysis,    Heterogeneous Populations,    Cell Culture,    Hippocampus,    MRNA Profiles,    Molecular Differences,    Cell Populations,    Harvesting Cells,    Electrophysiology,    Eppendorf Tube,    Molecular Biology Techniques,    ARNA Amplification,    Downstream Application,    Reverse-transcription,    Real-time Polymerase Chain Reaction (PCR),    Linear Amplification,    Polyadenylated RNA,   
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