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JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Journal
JoVE Encyclopedia of Experiments
神经科学
医学
免疫学与感染
遗传学
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癌症
生物学
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10.3791/67616-v
09:38 min
Fluorescence Labeling to Visualize Low-Expressed Proteins in Zebrafish
10.3791/67469-v
09:29 min
Electroporation-Based CRISPR-Cas9-Mediated Gene Knockout in THP-1 Cells and Single-Cell Clone Isolation
10.3791/66966-v
12:08 min
Retroviral CRISPR/Cas9-Mediated Gene Targeting for the Study of Th17 Differentiation in Vitro
10.3791/66581-v
05:30 min
CRISPR-Based Modular Assembly for High-Throughput Construction of a UAS-cDNA/ORF Plasmid Library
10.3791/65264-v
04:51 min
High-Efficiency Gene Disruption in Primary Bone Marrow-Derived Macrophages Using Electroporated Cas9-sgRNA Complexes
10.3791/64849-v
09:52 min
Investigation of Beige Fat Biology and Metabolism Using the CRISPR SunTag-p65-HSF1 Activation System
10.3791/64729-v
07:23 min
One-step CRISPR-based Strategy for Endogenous Gene Tagging in Drosophila melanogaster
10.3791/64209-v
06:52 min
CRISPR-Cas9-Mediated Precise Knock-In Edits in Zebrafish Hearts
10.3791/64195-v
08:19 min
Protocols for CRISPR/Cas9 Mutagenesis of the Oriental Fruit Fly Bactrocera dorsalis
10.3791/64064-v
08:32 min
CRISPR/Cas9 Gene Editing of Hematopoietic Stem and Progenitor Cells for Gene Therapy Applications
10.3791/63177-v
10:08 min
Determining the Role of Maternally-Expressed Genes in Early Development with Maternal Crispants
10.3791/62328-v
11:32 min
Gene Knock-in by CRISPR/Cas9 and Cell Sorting in Macrophage and T Cell Lines
10.3791/62245-v
Delivery of the Cas9/sgRNA Ribonucleoprotein Complex in Immortalized and Primary Cells via Virus-like Particles (“Nanoblades”)
10.3791/62068-v
06:37 min
Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera (Hübner)
10.3791/61855-v
08:20 min
Genome Engineering of Primary Human B Cells Using CRISPR/Cas9
10.3791/59780-v
09:05 min
Pooled CRISPR-Based Genetic Screens in Mammalian Cells
10.3791/59639-v
05:51 min
Construction of CRISPR Plasmids and Detection of Knockout Efficiency in Mammalian Cells through a Dual Luciferase Reporter System
10.3791/59382-v
10:10 min
HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries
10.3791/59202-v
Using Sniper-Cas9 to Minimize Off-target Effects of CRISPR-Cas9 Without the Loss of On-target Activity Via Directed Evolution
10.3791/58790-v
09:13 min
Generation of Cancer Cell Clones to Visualize Telomeric Repeat-containing RNA TERRA Expressed from a Single Telomere in Living Cells
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