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One-step CRISPR-based Strategy for Endogenous Gene Tagging in Drosophila melanogaster 10.3791/64729-v 07:23 min
One-step CRISPR-based Strategy for Endogenous Gene Tagging in Drosophila melanogaster
Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae 10.3791/64240-v 07:55 min
Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae
Gene Knock-in by CRISPR/Cas9 and Cell Sorting in Macrophage and T Cell Lines 10.3791/62328-v 11:32 min
Gene Knock-in by CRISPR/Cas9 and Cell Sorting in Macrophage and T Cell Lines
A Non-Coding Small RNA MicC Contributes to Virulence in Outer Membrane Proteins in Salmonella Enteritidis 10.3791/61808-v 06:30 min
A Non-Coding Small RNA MicC Contributes to Virulence in Outer Membrane Proteins in Salmonella Enteritidis
A Bioinformatics Pipeline for Investigating Molecular Evolution and Gene Expression using RNA-seq 10.3791/61633-v 07:09 min
A Bioinformatics Pipeline for Investigating Molecular Evolution and Gene Expression using RNA-seq
Rapid, Seamless Generation of Recombinant Poxviruses using Host Range and Visual Selection 10.3791/61049-v 09:25 min
Rapid, Seamless Generation of Recombinant Poxviruses using Host Range and Visual Selection
Markerless Gene Deletion by Floxed Cassette Allelic Exchange Mutagenesis in Chlamydia trachomatis 10.3791/60848-v 10:35 min
Markerless Gene Deletion by Floxed Cassette Allelic Exchange Mutagenesis in Chlamydia trachomatis
Preparation of Meiotic Chromosome Spreads from Zebrafish Spermatocytes 10.3791/60671-v 08:46 min
Preparation of Meiotic Chromosome Spreads from Zebrafish Spermatocytes
CRISPR-mediated Genome Editing of the Human Fungal Pathogen Candida albicans 10.3791/58764-v
CRISPR-mediated Genome Editing of the Human Fungal Pathogen Candida albicans
Identification of Homologous Recombination Events in Mouse Embryonic Stem Cells Using Southern Blotting and Polymerase Chain Reaction 10.3791/58467-v 08:01 min
Identification of Homologous Recombination Events in Mouse Embryonic Stem Cells Using Southern Blotting and Polymerase Chain Reaction
Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells 10.3791/55903-v 11:35 min
Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells
Rapid Deletion Production in Fungi via Agrobacterium Mediated Transformation of OSCAR Deletion Constructs 10.3791/55239-v 11:24 min
Rapid Deletion Production in Fungi via Agrobacterium Mediated Transformation of OSCAR Deletion Constructs
Conjugative Mating Assays for Sequence-specific Analysis of Transfer Proteins Involved in Bacterial Conjugation 10.3791/54854-v 10:41 min
Conjugative Mating Assays for Sequence-specific Analysis of Transfer Proteins Involved in Bacterial Conjugation
Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical Production 10.3791/54371-v 10:10 min
Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical Production
Biolistic Transformation of a Fluorescent Tagged Gene into the Opportunistic Fungal Pathogen Cryptococcus neoformans 10.3791/52666-v 07:32 min
Biolistic Transformation of a Fluorescent Tagged Gene into the Opportunistic Fungal Pathogen Cryptococcus neoformans
Efficient Generation of hiPSC Neural Lineage Specific Knockin Reporters Using the CRISPR/Cas9 and Cas9 Double Nickase System 10.3791/52539-v 14:46 min
Efficient Generation of hiPSC Neural Lineage Specific Knockin Reporters Using the CRISPR/Cas9 and Cas9 Double Nickase System
Subcloning Plus Insertion (SPI) – A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors 10.3791/52155-v
Subcloning Plus Insertion (SPI) – A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors
Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9 10.3791/52118-v
Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9
Embolic Middle Cerebral Artery Occlusion (MCAO) for Ischemic Stroke with Homologous Blood Clots in Rats 10.3791/51956-v 09:11 min
Embolic Middle Cerebral Artery Occlusion (MCAO) for Ischemic Stroke with Homologous Blood Clots in Rats
Rapid Synthesis and Screening of Chemically Activated Transcription Factors with GFP-based Reporters 10.3791/51153-v
Rapid Synthesis and Screening of Chemically Activated Transcription Factors with GFP-based Reporters
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