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JoVE Core
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JoVE Journal
JoVE Encyclopedia of Experiments
神经科学
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免疫学与感染
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09:44 min
3.10: Rapid Amplification of cDNA Ends
11:21 min
6.12: Phage Transduction: A Method to Transfer Ampicillin Resistance from Donor to Recipient E. coli
12:52 min
6.11: Conjugation: A Method to Transfer Ampicillin Resistance from Donor to Recipient E. coli
10:54 min
6.10: Transformation of E. coli Cells Using an Adapted Calcium Chloride Procedure
13:00 min
6.9: Plaque Assay: A Method to Determine Viral Titer as Plaque Forming Units (PFU)
11:33 min
6.8: Microscopy and Staining: Gram, Capsule, and Endospore Staining
13:39 min
6.7: Antibiotic Susceptibility Testing: Epsilometer Tests to Determine MIC Values of Two Antibiotics and Evaluate Antibiotic Synergy
12:15 min
6.6: Growth Curves: Generating Growth Curves Using Colony Forming Units and Optical Density Measurements
10:57 min
6.5: 16S rRNA Sequencing: A PCR-based Technique to Identify Bacterial Species
09:03 min
6.4: Pure Cultures and Streak Plating: Isolation of Single Bacterial Colonies from a Mixed Sample
09:35 min
6.3: Enrichment Cultures: Culturing Aerobic and Anaerobic Microbes on Selective and Differential Medias
10:53 min
6.2: Serial Dilutions and Plating: Microbial Enumeration
08:08 min
6.1: Creating a Winogradsky Column: A Method to Enrich the Microbial Species in a Sediment Sample
11:48 min
5.12: Assay for Cell Death: Chromium Release Assay of Cytotoxic Ability
11:04 min
5.11: Adoptive Cell Transfer: Introducing Donor Mouse Splenocytes to a Host Mouse and Assessing Success via FACS
10:57 min
5.10: Cell Cycle Analysis: Assessing CD4 and CD8 T Cell Proliferation After Stimulation Using CFSE Staining and Flow Cytometry
13:46 min
5.9: Immunoprecipitation-Based Techniques: Purification of Endogenous Proteins Using Agarose Beads
13:13 min
5.8: Confocal Fluorescence Microscopy: A Technique to Determine the Localization of Proteins in Mouse Fibroblasts
09:56 min
5.7: Immunofluorescence Microscopy: Immunofluorescence Staining of Paraffin-Embedded Tissue Sections
13:21 min
5.6: Antibody Generation: Producing Monoclonal Antibodies Using Hybridomas
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